We report that the rate of synapse development in primary sensory cortices of mice and macaques is unrelated to lifespan, as was previously thought. Automatic segmentation of neurons, synapses and mitochondria of mouse V1 L4 p14 and p105 are available as a WebKnossos format available for further public proofreading/error checking. Mouse p36 datasets are publicly available here for L2/3: and here for L4. ![]() Mouse somatosensory cortex(6×6×30 cubic nanometer resolution). ![]() Adult S1 excitatory synapse measurements were manually analyzed using the publicly available neuroglancer file from which can be found here: Kasthuri et al., 2014. Mouse S1 datasets that we analyzed for changes in excitatory synapses are available through original publication. All original EM data for V1 are freely available online at. Homemade code used for data analysis of Knossos traced skeletons (i.e., xml files) can be freely accessed here. ![]() 3D alignment was performed using the publicly available Aligntk software available here. ![]() Homemade code used for EM image processing (i.e., 2D stitching, 3D alignments, brightness/contrast normalization) can be freely accessed here.
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